Journal: Arthritis Research & Therapy

Article Title: IL-17 sequestration via salivary gland gene therapy in a mouse model of Sjogren’s syndrome suppresses disease-associated expression of the putative autoantigen Klk1b22

doi: 10.1186/s13075-015-0714-2

Figure Lengend Snippet: Identification and relative quantification of the putative biomarkers Kallikrein-1 and Klk1b22 after treatment with IL-17R:Fc gene therapy. a Mass spectrometry sequence coverage of spots 6, 9 and 10 identified as Klk1b22. b Gel imaging shown in Fig. was subjected to relative quantitative analysis to measure fluorescent intensity of spots positively identified to be Kallikrein-1 (4 and 5) or Klk1b22 (6, 9, and 10). Numerical values of these intensities are shown in Table . c Magnification of 2-D gels run with either ultrasound-assisted gene transfer/Luciferase ( UAGT/Luc ) or UAGT/IL-17R:Fc samples, showing total Kallikrein-1 (KLK1, top bars ) and phosphorylated Kallikrein-1 ( bottom bars ). Acidic shift of KLK1 is attributed to phosphorylation

Article Snippet: Samples were cleaned using a 2-D Quant kit (GE Healthcare) and resuspended in 2D gel rehydration buffer (7 M Urea, 2 M Thiourea, 2 % CHAPS) containing 50 mM Tris pH8.5.

Techniques: Mass Spectrometry, Sequencing, Imaging, Luciferase